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diff-quik fixative solution  (Sysmex Corporation)

 
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    Sysmex Corporation diff-quik fixative solution
    Diff Quik Fixative Solution, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/diff-quik+fixative/diff+quik/pm38734854-73-19-30
    Average 90 stars, based on 1 article reviews
    diff-quik fixative solution - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: High Prevalence of Human Liver Infection by Amphimerus spp. Flukes, Ecuador
    Article Snippet: Recovered worms were fixed with 10% formalin, stained with Diff-Quik fixative (Sysmex, Kobe, Japan), and identified by comparing their morphologic features to known adult Clonorchis and Opisthorchis spp. worms.

    Article Title: Helicobacter pylori CagA promotes Snail-mediated epithelial-mesenchymal transition by reducing GSK-3 activity.
    Article Snippet: After 24 h incubation of the transwell system at 37 C, the cells that had migrated through the membrane were fixed with Diff-Quik fixative (Sysmex), stained with Diff-Quik solution, and counted from five independent sites.

    Article Title: HIF1A overexpression using cell-penetrating DNA-binding protein induces angiogenesis in vitro and in vivo.
    Article Snippet: Hypoxia-inducible factor-1 alpha (HIF1A) is an important transcription factor for angiogenesis.. Recent studies have used the protein transduction domain (PTD) to deliver genes, but the PTD has not been used to induce the expression of HIF1A.. This study aimed at using a novel PTD (Hph-1-GAL4; ARVRRRGPRR) to overexpress the HIF1A and identify the effects on angiogenesis in vitro and in vivo.

    Diff-Quik:

    Article Title: High Prevalence of Human Liver Infection by Amphimerus spp. Flukes, Ecuador
    Article Snippet: Recovered worms were fixed with 10% formalin, stained with Diff-Quik fixative (Sysmex, Kobe, Japan), and identified by comparing their morphologic features to known adult Clonorchis and Opisthorchis spp. worms.

    Article Title: Helicobacter pylori CagA promotes Snail-mediated epithelial-mesenchymal transition by reducing GSK-3 activity.
    Article Snippet: After 24 h incubation of the transwell system at 37 C, the cells that had migrated through the membrane were fixed with Diff-Quik fixative (Sysmex), stained with Diff-Quik solution, and counted from five independent sites.

    Article Title: HIF1A overexpression using cell-penetrating DNA-binding protein induces angiogenesis in vitro and in vivo.
    Article Snippet: Hypoxia-inducible factor-1 alpha (HIF1A) is an important transcription factor for angiogenesis.. Recent studies have used the protein transduction domain (PTD) to deliver genes, but the PTD has not been used to induce the expression of HIF1A.. This study aimed at using a novel PTD (Hph-1-GAL4; ARVRRRGPRR) to overexpress the HIF1A and identify the effects on angiogenesis in vitro and in vivo.

    Staining:

    Article Title: High Prevalence of Human Liver Infection by Amphimerus spp. Flukes, Ecuador
    Article Snippet: Recovered worms were fixed with 10% formalin, stained with Diff-Quik fixative (Sysmex, Kobe, Japan), and identified by comparing their morphologic features to known adult Clonorchis and Opisthorchis spp. worms.

    Article Title: Helicobacter pylori CagA promotes Snail-mediated epithelial-mesenchymal transition by reducing GSK-3 activity.
    Article Snippet: After 24 h incubation of the transwell system at 37 C, the cells that had migrated through the membrane were fixed with Diff-Quik fixative (Sysmex), stained with Diff-Quik solution, and counted from five independent sites.

    Article Title: HIF1A overexpression using cell-penetrating DNA-binding protein induces angiogenesis in vitro and in vivo.
    Article Snippet: Hypoxia-inducible factor-1 alpha (HIF1A) is an important transcription factor for angiogenesis.. Recent studies have used the protein transduction domain (PTD) to deliver genes, but the PTD has not been used to induce the expression of HIF1A.. This study aimed at using a novel PTD (Hph-1-GAL4; ARVRRRGPRR) to overexpress the HIF1A and identify the effects on angiogenesis in vitro and in vivo.

    Membrane:

    Article Title: High Prevalence of Human Liver Infection by Amphimerus spp. Flukes, Ecuador
    Article Snippet: Recovered worms were fixed with 10% formalin, stained with Diff-Quik fixative (Sysmex, Kobe, Japan), and identified by comparing their morphologic features to known adult Clonorchis and Opisthorchis spp. worms.

    Article Title: Helicobacter pylori CagA promotes Snail-mediated epithelial-mesenchymal transition by reducing GSK-3 activity.
    Article Snippet: After 24 h incubation of the transwell system at 37 C, the cells that had migrated through the membrane were fixed with Diff-Quik fixative (Sysmex), stained with Diff-Quik solution, and counted from five independent sites.

    Article Title: HIF1A overexpression using cell-penetrating DNA-binding protein induces angiogenesis in vitro and in vivo.
    Article Snippet: Hypoxia-inducible factor-1 alpha (HIF1A) is an important transcription factor for angiogenesis.. Recent studies have used the protein transduction domain (PTD) to deliver genes, but the PTD has not been used to induce the expression of HIF1A.. This study aimed at using a novel PTD (Hph-1-GAL4; ARVRRRGPRR) to overexpress the HIF1A and identify the effects on angiogenesis in vitro and in vivo.



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    Image Search Results


    Activation of phagocytosis by NETs. Neutrophils were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of Giemsa-stained neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.

    Journal: Frontiers in Immunology

    Article Title: Neutrophil Extracellular Traps Activate Proinflammatory Functions of Human Neutrophils

    doi: 10.3389/fimmu.2021.636954

    Figure Lengend Snippet: Activation of phagocytosis by NETs. Neutrophils were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of Giemsa-stained neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.

    Article Snippet: The preparations contained >99% granulocytes, of which >96% were neutrophils and 1%–4% were eosinophils, as determined by Giemsa staining (Diff Quik ® Fix, Medion Diagnostics, Berlin, Germany) of cytocentrifuged (Shandon) samples ( ).

    Techniques: Activation Assay, Flow Cytometry, Staining, Fluorescence, Microscopy